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李少伟教授博导

邮  箱:shaowei@xmu.edu.cn

职称/职务:教授 博士生导师

联系方式:+86-592-2880620

  • 个人简介
  • 科研领域
  • 代表性成果

1998年,澳门大阳城集团生物化学专业,学士学位;
2003年,澳门大阳城集团生化与分子生物学/动物学专业,博士学位;
2003年至今,澳门大阳城集团澳门大阳城集团2138网站,讲师/副教授/教授。
B.S. 1998, Xiamen University, Biochemistry;
Ph.D. 2003, Xiamen University, Biochemistry & Molecular Biology/Zoology;
Lecturer/Associate Professor/Professor, School of Life Sciences, Xiamen University, 2003 to Present.

基因工程疫苗研究与开发;
重要传染病病毒免疫表位的结构和功能研究;
基于结构的分子模拟和分子设计。
Research and development of recombinant human vaccines;
Structural basis and functional implication for underlying immune epitopes of key infectious viruses;
Structure-based rational design for vaccine immunogens.

代表性论文(# co-first author, * Corresponding author):

1. Ju B#, Zheng Q#, Guo H#, Fan Q#, Li T#, Song S#, Sun H#, Shen S, Zhou X, Xue W, Cui L, Zhou B, Li S*, Xia N*, Zhang Z*. Immune escape by SARS-CoV-2 Omicron variant and structural basis of its effective neutralization by a broad neutralizing human antibody VacW-209. Cell Res. 2022 May;32(5):491-494.
2. Wang S#, Sun H#, Zhang Y#, Yuan L#, Wang Y#, Zhang T#, Wang S, Zhang J, Yu H, Xiong H, Tang Z, Liu L, Huang Y, Chen X, Li T, Ying D, Liu C, Chen Z, Yuan Q, Zhang J*, Cheng T*, Li S*, Guan Y*, Zheng Q*, Zheng Z*, Xia N*. Three SARS-CoV-2 antibodies provide broad and synergistic neutralization against variants of concern, including Omicron. Cell Rep. 2022 May 24;39(8):110862.
3. Wang G#, Zha Z#, Huang P#, Sun H#, Huang Y, He M, Chen T, Lin L, Chen Z, Kong Z, Que Y, Li T, Gu Y, Yu H, Zhang J, Zheng Q*, Chen Y*, Li S*, Xia N*. Structures of pseudorabies virus capsids. Nat Commun. 2022 Mar 22;13(1):1533.
4. Liu L#, Chen T#, Zhou L, Sun J, Li Y, Nie M, Xiong H, Zhu Y, Xue W, Wu Y, Li T, Zhang T, Kong Z, Yu H, Zhang J, Gu Y, Zheng Q, Zhao Q*, Xia N, Li S*. A Bacterially Expressed SARS-CoV-2 Receptor Binding Domain Fused With Cross-Reacting Material 197 A-Domain Elicits High Level of Neutralizing Antibodies in Mice. Front Microbiol. 2022 Apr 26;13:854630.
5. Huang Y#, Li Z#, Hong Q, Zhou L, Ma Y, Hu Y, Xin J, Li T, Kong Z, Zheng Q, Chen Y, Zhao Q, Gu Y, Zhang J, Wang Y*, Yu H*, Li S*, Xia N. A stepwise docking molecular dynamics approach for simulating antibody recognition with substantial conformational changes. Comput Struct Biotechnol J. 2022 Jan 18;20:710-720.
6. T Li#, W Xue#, Q Zheng#, S Song#, C Yang#, H Xiong#, S Zhang, M Hong, Y Zhang, H Yu, Y Zhang, H Sun, Y Huang, T Deng, X Chi, J Li, S Wang, L Zhou, T Chen, Y Wang, T Cheng, T Zhang, Q Yuan, Q Zhao, J Zhang, J. S. McLellan, Z Zhou*, Z Zhang*, S Li*, Y Gu*, N Xia*. Cross-neutralizing antibodies bind a SARS-CoV-2 cryptic site and resist circulating variants. Nat Commun, 2021, 12(1): 5652-5664.
7. L Xu#, Q Zheng#, R Zhu#, Z Yin#, H Yu#, Y Lin, Y Wu, M He, Y Huang, Y Jiang, H Sun, Z Zha, H Yang, Q Huang, D Zhang, Z Chen, X Ye, J Han, L Yang, C Liu, Y Que, M Fang, Y Gu, J Zhang, W Luo, Z Zhou*, S Li*, T Cheng*, N Xia*. Cryo-EM Structures Reveal Basis of Receptor-Initiated Coxsackievirus Uncoating with Implications for Therapeutic Antibody Development. Cell Host Microbe, 2021, S1931-3128(21)00001-9.
8. M He#, C Xin#, Z Zha#, Y Li, J Chen, Y Huang, S Huang, M Yu, Z Wang, S Song, X Liu, S Wei, Z Li, T Li, Y Wang, HYu, Q Zhao, J Zhang, Q Zheng*, Y Gu*, S Li*, N Xia*. Structural basis for the shared neutralization mechanism of three classes of human papillomavirus type 58 antibodies with disparate modes of binding. J Virol, 2021, 01587-20.
9. W Wang#, Q Zheng#, D Pan#, H Yu#, W Fu, J Liu, M He, R Zhu, Y Cai, Y Huang, Z Zha, Z Chen, X Ye, J Han, Y Que, T Wu, J Zhang, S Li*, H Zhu*,Z Zhou*,T Cheng*and N Xia*. Near-atomic cryo-electron microscopy structures of varicella-zoster virus capsids. Nat Microbiol, 2020,5(12) :1542-1552.
10. M He#, L Xu#, Q Zheng#, R Zhu#, Z Yin, Z Zha, Y Lin, L Yang, Y Huang, X Ye, S Li, W Hou, Y Wu, J Han, D Liu, Z Li, Z Chen, H Yu, Y Que, Y Wang, X Yan, J Zhang, Y Gu, Z Zhou*, T Cheng*, S Li*, N Xia*. Identification of Antibodies with Non-overlapping Neutralization Sites that Target Coxsackievirus A16. Cell Host Microbe, 2020, 27(2): 249-261.
11. D Wang#, X Liu#, M Wei#, C Qian, S Song, J Chen, Z Wang, Q Xu, Y Yang, M He, X Chi, S Huang, T Li, Z Kong, Q Zheng, H Yu, Y Wang, Q Zhao, J Zhang, N Xia*, Y Gu*& S Li*. Rational design of a multi-valent human papillomavirus vaccine by capsomere-hybrid co-assembly of virus-like particles. Nat Commun, 2020, 11(1):2841.
12. T Li#, Q Zheng*#, H Yu, D Wu, W Xue, H Xiong, X Huang, M Nie, M Yue, R Rong, S Zhang, Y Zhang, Y Wu, S Wang, Z Zha, T Chen, T Deng, Y Wang, T Zhang, Y Chen, Q Yuan*, Q Zhao, J Zhang, Y Gu*, S Li*, N Xia*. SARS-CoV-2 spike produced in insect cells elicits high neutralization titres in non-human primates. Emerg Microbes Infect, 2020; 9(1): 2076–2090
13. W Huang#, M He#, T Ning, J Nie, F Zhang, Q Zheng, R Zhang, Y Xu, Y Gu, S Li*, Y Wang*. Structural characterization of a neutralizing mAb H16.001 that is qualified to a common potency assay for various HPV16 vaccines. NPJ Vaccines, 2020, 5:89.
14. C Qian, X Liu, Q Xu, Z Wang, J Chen, T Li, Q Zheng, H Yu, Y Gu*, S Li*, N Xia*. Recent Progress on the Versatility of Virus-Like Particles. Vaccines,2020, 20;8(1):139.
15. L Zhou#, H Yu#, K Wang#, T Chen, Y Ma, Y Huang, J Li, L Liu, Y Li, Z Kong, Q Zheng, Y Wang, Y Gu, N Xia, S Li*. Genome re-sequencing and reannotation of the Escherichia coli ER2566 strain and transcriptome sequencing under overexpression conditions. BMC Genomics, 2020, 21(1):407.
16. Q Zheng#, R Zhu#, L Xu#, M He#, X Yan, D Liu, Z Yin, Y Wu, Y Li, L Yang, W Hou, S Li, Z Li, Z Chen, Z Li, H Yu, Y Gu, J Zhang, T. S. Baker, Z Zhou, B. S. Graham*, T Cheng*, S Li*, N Xia*. Atomic structures of enterovirus D68 in complex with two monoclonal antibodies define distinct mechanisms of viral neutralization. Nat Microbiol, 2019, 4(1): 124-133.
17. Q Zheng#, J Jiang#, M He#, Z Zheng, H Yu, T Li, W Xue, Z Tang, D Ying, Z Li, S Song, X Liu, K Wang, Z Zhang, D Wang, Y Wang, X Yan, Q Zhao, J Zhang, Y Gu*, S Li*, N Xia*. Viral neutralization by antibody-imposed physical disruption. Proc Natl Acad Sci U S A, 2019, 116(52): 26933-26940.
18. X Liu#, J Chen#, Z Wang, D Wang, M He, C Qian, S Song, X Chi, Z Kong, Q Zheng, Y Wang, H Yu, Q Zhao, J Zhang, S Li*, Y Gu*, N Xia*. Neutralization sites of human papillomavirus-6 relate to virus attachment and entry phase in viral infection. Emerg Microbes Infect, 2019, 8(1): 1721-1733.
19. T Chen#, K Wang#, X Chi, L Zhou, J Li, L Liu, Q Zheng, Y Wang, H Yu, Y Gu, J Zhang, S Li*, N Xia*. Construction of a bacterial surface display system based on outer membrane protein F. Microb Cell Fact, 2019, 18: 0-70.
20. K Wang#, L Zhou#, X Zhang, C Song, T Chen, J Li, M Zheng, Y Wang, Q Zheng, Z Zheng, H Yu, T Wu, Y Gu, J Zhang, Q Zhao*, S Li*, N Xia*. Hepatitis E vaccine candidate harboring a non-particulate immunogen of E2 fused with CRM197 fragment A. Antiviral Res, 2019(164): 154-161.
21. T Li, Z Zhang, J Qiao, R Rong, Y Zhang, Q Yao, Z Li, H Shen, F Huang, W Xue, S Gao, S Li, Q Zheng, H Yu, J Zhang, Y Gu *, S Li*, N Xia. Characterization of native-like HIV-1 gp140 glycoprotein expressed in insect cells. Vaccine, 2019, Feb, 37(11): 1418-1427.
22. Z Li#, S Song#, M He#, D Wang, J Shi, X Liu, Y Li, X Chi, S Wei, Y Yang, Z Wang, J Li, H Qian, H Yu, Q Zheng, X Yan, Q Zhao, J Zhang, Y Gu*, S Li*, N Xia*. Rational design of a triple-type human papillomavirus vaccine by compromising viral-type specificity. Nat Commun, 2018, 9(1): 0-5360.
23. R Zhu#, L Xu#, Q Zheng#, Y Cui#, S Li#, M He, Z Yin, D Liu, S Li, Z Li, Z Chen, H Yu, Y Que , C Liu, Z Kong, J Zhang, T. S. Baker, X Yan*, Z Zhou*, T Cheng*, N Xia*. Discovery and structural characterization of a therapeutic antibody against coxsackievirus A10. Sci Adv, 2018, 4(9): 0-eaat7459.
24. Z Zhang#, M He#, S Bai, F Zhang, J Jiang, Q Zheng, S Gao, X Yan, S Li*, Y Gu*, N Xia. T = 4 Icosahedral HIV-1 Capsid As an Immunogenic Vector for HIV-1 V3 Loop Epitope Display. Viruses, 2018, 10(12).
25. M Wei#, D Wang#, Z Li, S Song, X Kong, X Mo, Y Yang, M He, Z Li, B Huang, Z Lin, H Pan, Q Zheng, H Yu, Y Gu, J Zhang, S Li*, N Xia*. N-terminal truncations on L1 proteins of human papillomaviruses promote their soluble expression in Escherichia coli and self-assembly in vitro. Emerg Microbes Infect, 2018, 7(1): 0-160.
26. Q Lin#, T Li#, Y Chen#, S. Y. Lau, M Wei, Y Zhang, Z Zhang, Q Yao, J Li, Z Li, D Wang, Q Zheng, H Yu, Y Gu, J Zhang, H Chen, S Li*, N Xia*. Structural Basis for the Broad, Antibody-Mediated Neutralization of H5N1 Influenza Virus. J Virol, 2018, 92(17): e00547-18.
27. M Zheng, J Jiang, X Zhang, N Wang, K Wang, Q Li, T Li, Q Lin, Y Wang, H Yu, Y Gu, J Zhang, S Li*, N Xia. Characterization of capsid protein (p495) of hepatitis E virus expressed ID in Escherichia coli and assembling into particles in vitro. Vaccine, 2018, 36(16): 2104-2111.
28. Z Li#, D Wang#, Y Gu#, S Song, M He, J Shi, X Liu, S Wei, J Li, H Yu, Q Zheng, X Yan, T. S. Baker, J Zhang, J. S. McLellan*, S Li*, N Xia*. Crystal Structures of Two Immune Complexes Identify Determinants for Viral Infectivity and Type-Specific Neutralization of Human Papillomavirus. MBio, 2017, 8(5).
29. L Xu#, Q Zheng#, S Li#, M He#, Y Wu, Y Li, R Zhu, H Yu, Q Hong, J Jiang, Z Li, S Li, H Zhao, L Yang, W Hou, W Wang, X Ye, J Zhang, T. S. Baker, T Cheng *, Z Zhou, X Yan*, N Xia*. Atomic structures of Coxsackievirus A6 and its complex with a neutralizing antibody. Nat Commun. 2017 Sep 11;8(1):505.
30. H Pan, Z Li, J Wang, S Song, D Wang, M Wei, Y Gu, J Zhang, S Li*, N Xia*. Bacterially expressed human papillomavirus type 6 and 11 bivalent vaccine: Characterization, antigenicity and immunogenicity. Vaccine, 2017, 35(24):3222-3231.
31. Y Gu, M Wei, D Wang, Z Li, M Xie, H Pan, T Wu, J Zhang, S Li*, N Xia*. Characterization of an Escherichia coil-derived human papillomavirus type 16 and 18 bivalent vaccine. Vaccine, 2017, 35(35): 4637-4645.
32. D Wang#, F Fan#, Z Li, X Liu, S Song, S Wei, M He, Y Lin, Z Li, M Wei, H Yu, Y Gu, S Li*, N Xia. Stop codon mutagenesis for homogenous expression of human papillomavirus L1 protein in Escherichia coli. Protein expr and purif, 2017, 133: 110-120.
33. Gu Y, Wei M, Wang D, Li Z, Xie M, Pan H, Wu T, Zhang J, Li S*, Xia N*. Characterization of an Escherichia coli-derived human papillomavirus type 16 and 18 bivalent vaccine. Vaccine. 2017 Aug 16;35(35 Pt B):4637-4645.
34. Z Li#, X Yan#, H Yu#, D Wang, S Song, Y Li, M He, Q Hong, Q Zheng, Q Zhao, Y Gu, J Zhang, M. E. Janssen, G Cardone, N. H. Olson, T. S. Baker*, Li S*, Xia N. The C-Terminal Arm of the Human Papillomavirus Major Capsid Protein Is Immunogenic and Involved in Virus-Host Interaction. Structure. 2016 Jun 7;24(6):874-85.
35. X Zhang, K Wang, Q Lin, M Zheng, Q Li, T Li, Q Hong, Q Zheng, H Yu *, Y Gu, Li S*, Xia N*. A shared N-terminal hydrophobic tail for the formation of nanoparticulates. Nanomedicine (Lond) . 2016 Sep;11(17):2289-303.
36. Q Lin, K Yang, F He, J Jiang, T Li, Z Chen, R Li, Y Chen, Li S*, Q Zhao*, N Xia. Production of Influenza Virus HA1 Harboring Native-Like Epitopes by Pichia pastoris. Appl Biochem Biotechnol. 2016 Aug;179(7):1275-89.
37. Y Gu, X Tang, X Zhang, C Song, M Zheng, K Wang, J Zhang, M. H. Ng, C. L. Hew, S Li*, N Xia*, J Sivaraman*. Structural basis for the neutralization of hepatitis E virus by a cross-genotype antibody. Cell Res. 2015 May, 25(5): 604-20.
38. S Li1, J Zhang, N Xia*. Lessons from hepatitis E vaccine design. Curr Opin Virol. 2015 Apr, 11:130-136.
39. S Li1, Q Zhao, T Wu*, S Chen, J Zhang, Xia, N Xia. The development of a recombinant hepatitis E vaccine HEV 239. Hum Vaccin Immunother, 2015, 11(4): 908-914.
40. D Wang, Z Li, J Xiao, J Wang, L Zhang, Y Liu, F Fan, L Xin, M Wei, Z Kong, H Yu, Y Gu, J Zhang, S Li *, N Xia*. Identification of Broad-Genotype HPV L2 Neutralization Site for Pan-HPV Vaccine Development by a Cross-Neutralizing Antibody. PLoS One, 2015 Apr 23, 10(4):e0123944.
41. Y Gu, C Fang, W Lei, W Hou, J Zhang, C. l. Hew, S Li*, Y. A. Yuan*, Ningshao Xia*; Structure of a Novel Shoulder-to-Shoulder p24 Dimer in Complex with the Broad-Spectrum Antibody A10F9 and Its Implication in Capsid Assembly. PLoS One, 2013, 8(4):e61314.
42. C Yang, H Pan, M Wei, X Zhang, N Wang, Y Gu, H Du, J Zhang, S Li*, N Xia*. Hepatitis E virus capsid protein assembles in 4M urea in the presence of salts. Protein Sci. 2013 Mar, 22(3):314-26.
43. X Tang, C Yang, Y Gu, C Song, X Zhang, Y Wang, J Zhang, C. L. Hew, S Li*, N Xia*, J Sivaraman *. Structural basis for the neutralization and genotype specificity of hepatitis E virus. Proc Natl Acad Sci U S A. 2011, 108(25):10266-71.
44. H Yu, S Li*, C Yang, M Wei, C Song, Z Zheng, Y Gu, H Du, J Zhang, N Xia. Homology model and potential virus-capsid binding site of a putative HEV receptor Grp78. J Mol Model, 2011, 17(5): 987-995.
45. K Yang, S Li*, F He, J Zhang, M Wei, H Pan, C Yang, J Xiong, J Zhang, N Xia. Expression and Purification of Soluble HIV-1 Envelope Glycoprotein gp160 Mutant from Saccharomyces Cerevisiae. J Biosci Bioeng, 2009 Jul, 108(1):5-10.
46. S Li#, X Tang#, J Seetharaman#, C Yang, Y Gu, J Zhang, H Du, J. W. Shih, C. L. Hew, J Sivaraman*, N Xia*. Dimerization of Hepatitis E Virus Capsid Protein E2s Domain is Essential for Virus-Host Interaction. PLoS Pathog, 2009 Aug, 5(8):e1000537.
47. S Li, J Zhang, Y Li, S Ou, G Huang, Z He, S Ge, Y Xian, S Pang, M. H. Ng, N Xia*. A bacterially expressed particulate hepatitis E vaccine: antigenicity, immunogenicity and protectivity on primates. Vaccine, 2005 Apr 22, 23(22):2893-2901.
48. S Li, J Zhang, Z He, Y Gu, R Liu, J Lin, Y Chen, M. H. Ng, N Xia*. Mutational analysis of essential interactions involved in the assembly of hepatitis E virus capsid. J Biol Chem, 2005 Feb 4, 280(5):3400-6.

荣誉、奖励及参加学术团体的情况:

国家百千万人才工程“有突出贡献中青年专家”,2020年
担任世界卫生组织-疫苗产品开发咨询委员会成员,2020年
主持国家自然科学基金、“863”课题、新药创制重大专项及滚动项目等课题,2020年
中国科协求是杰出青年成果转化奖,2020年
“厦门市拔尖人才”,2019年
中国专利金奖和第十届紫金科技创新奖,2018年
《自然•生物技术》评选的2016年全球Top20转化研究者,是中国大陆首次入选的人员,2017年
作为主要成员研制出戊肝疫苗、宫颈癌疫苗、尖锐湿疣疫苗、戊肝诊断试剂、艾滋诊断试剂和新冠诊断试剂等新药,2017年
中国科协求是杰出青年学者奖,2015年
第十四届中国专利奖-金奖,2012年
教育部新世纪优秀人才,2011年
福建省杰出青年基金,2011年
国家科技进步二等奖,2001年
国家技术发明二等奖,2010年
中国高校科技进步一等奖,2001年
获授权发明专利29项,国际授权15项